Yeast cell detection using fuzzy automatic contrast ... - Nature
2023年9月27日 · Yeast cells, representing a vital component of the fungi family, hold profound significance in elucidating the intricacies of eukaryotic cells and human biology. The proposed methodology...
Yeast Secrete and Detect - Addgene
This kit contains a collection of 42 plasmids to enable a Golden Gate cloning-based one step-one pot assembly of transcription units for protein secretion in the yeast organisms Saccharomyces cerevisiae and Pichia pastoris. It contains a variety of methanol utilization (MUT) promoters, signal peptides and C-terminal tags as well as matching ...
Yeast-based biosensors: design and applications
2015年1月6日 · Biosensors incorporating various yeasts have been reported to detect an incredibly large range of molecules including but not limited to odorants, metals, intracellular metabolites, carcinogens, lactate, alcohols, and sugars.
Yeast identification — past, present, and future methods
2007年3月1日 · Invasive yeast infections are associated with high levels of morbidity and mortality, so rapid identification can contribute to successful therapy, earlier resolution of infections, and subsequent reduction of moribund outcomes.
Low false positive and accurate detection of yeast cell viability and ...
2020年5月7日 · In this work, we proposed an automatic staining and lensfree diffraction imaging method (FED method) to detect yeast cell viability and concentration based on the extracted energy distribution parameters of lensfree diffraction fingerprint.
Yeast-based biosensors: design and applications - PubMed
Yeast-based biosensing (YBB) is an exciting research area, as many studies have demonstrated the use of yeasts to accurately detect specific molecules. Biosensors incorporating various yeasts have been reported to detect an incredibly large range of …
Real-Time Detection of Yeast Growth on Solid Medium through
2024年4月26日 · Yeast (Pichia pastoris, strain GS115) is used as a model organism to demonstrate biosensor performance. The biosensor comprises an interdigital capacitor in the center with a helical inductive structure surrounding it.
A GPCR-based yeast biosensor for biomedical, biotechnological, …
2022年6月27日 · We construct a CB 2 receptor-based biosensor, optimize it to achieve high sensitivity and dynamic range, and prove its effectiveness in three applications of increasing difficulty. First, we screen...
Development of a yeast cell surface display method using the …
2021年5月26日 · Yeast cell surface display (YSD) has been used to engineer various proteins, including antibodies. Directed evolution, which subjects a gene to iterative rounds of mutagenesis,...
Development of multiplex loop-mediated isothermal ... - PubMed
We have developed loop-mediated isothermal amplification (LAMP) assays to detect the emerging opportunistic pathogenic yeasts: Candida albicans, Candida glabrata, Candida tropicalis, the Candida parapsilosis group, Trichosporon asahii, and Trichosporon mucoides. These yeasts may cause deep-seated candidiasis or trichosporonosis.