From rabbit antibody repertoires to rabbit monoclonal antibodies …
2017年3月24日 · Starting with the unique ontogeny of rabbit B cells that affords highly distinctive antibody repertoires rich in in vivo pruned binders of high diversity, affinity and specificity, we describe...
Recombinant rabbit anti-human SLC39A6/LIV-1 chimeric …
This recombinant rabbit monoclonal antibody is part of a collection of rabbit mAbs against druggable targets developed by direct cloning from immunized rabbit B cells without hybridoma fusion. All antibodies have been fully sequenced and …
Generation and Characterization of a Chimeric Rabbit/Human …
2010年4月2日 · Monoclonal antibody antigen-binding domains (Fabs) have proven useful for the co-crystallization of other refractory proteins. In the present study, a chimeric rabbit/human anti-Rev Fab was selected by phage display, expressed in a bacterial secretion system, and purified from the media. The Fab readily solubilized polymeric Rev.
Chimeric Rabbit/Human Fab and IgG Specific for Members of …
2007年1月1日 · Here we exploit the same immune repertoires by phage display technology to generate rabbit monoclonal antibodies (mAbs) with nanomolar affinity to epitopes that are specific for NgR1 and NgR2, respectively, but at the same time conserved between mouse, rat, and human orthologs.
Rabbits transgenic for human IgG genes recapitulating rabbit B …
2020年11月24日 · Through the knockout of endogenous IgM genes and the introduction of human immunoglobulin sequences, this rabbit strain has been engineered to generate a highly diverse human IgG antibody repertoire. We further incorporated human CD79a/b and Bcl2 (B-cell lymphoma 2) genes, which enhance B-cell receptor expression and B-cell survival.
Chimeric rabbit/human Fab antibodies against the hepatitis Be …
2017年10月6日 · Here, to further characterize HBeAg, we used phage display to produce a panel of chimeric rabbit/human monoclonal antibody fragments (both Fab and scFv) against rHBeAg. Several of the Fab/scFv, expressed in Escherichia coli , had unprecedentedly high binding affinities ( K d ∼10 -12 m) and high specificity.
chimeric rabbit/human monoclonal antibody fragments (both Fab and scFv) against rHBeAg. Several of the Fab/scFv, ex-pressed in Escherichia coli, had unprecedentedly high binding affinities (K d 10 12 M) and high specificity. We used Fab/scFv in the context of an enzyme-linked immunosorbent assay (ELISA) for HBeAg quantification, which we ...
Chimeric rabbit/human Fab antibodies against the hepatitis Be …
We have generated chimeric rabbit/human monoclonal antibody fragments directed against the rHBeAg through phage display. Rabbit monoclonal antibodies often have both high affinity and specificity and can recognize epitopes conserved between …
HA-Tag Chimeric Rabbit-Human Monoclonal Antibody (Clone …
HA-Tag Chimeric Rabbit-Human Monoclonal Antibody (Clone RMH02): For immunochemical detection of HA-tagged proteins. Synonym: Hemagglutinin. Supplier of assay kits, antibodies, biochemicals, and proteins and provider of contract research services.
Generation of Antibody Libraries for Phage Display: Chimeric Rabbit ...
Phage display is a powerful method for the de novo generation, affinity maturation, and humanization of rabbit monoclonal antibodies from naive, immune, and synthetic antibody repertoires. Using phagemid family pComb3, a preferred phage display format is chimeric rabbit/human Fab, which consists of rabbit variable domains (V H , V κ , and V λ ...