Quantitative tRNA-sequencing uncovers metazoan tissue-specific …
2020年8月14日 · Here we present Quantitative Mature tRNA sequencing (QuantM-tRNA seq), a technique to monitor tRNA abundance and sequence variants secondary to RNA modifications. With QuantM-tRNA seq, we...
Quantitative analysis of tRNA abundance and modifications by …
2023年4月6日 · Here we introduce Nano-tRNAseq, a nanopore-based approach to sequence native tRNA populations that provides quantitative estimates of both tRNA abundances and modification dynamics in a...
Advances in methods for tRNA sequencing and quantification
2024年3月1日 · This review article summarizes various approaches for tRNA-seq, a tool for studying tRNA abundance, modifications, and function. It compares the advantages and disadvantages of NGS-based and hybridization-based methods, and discusses the challenges and applications of tRNA-seq.
Advances in methods for tRNA sequencing and quantification
2023年12月19日 · In the past decade tRNA sequencing (tRNA-seq) has attracted considerable attention as an important tool for the development of novel approaches to quantify highly modified tRNA species and to propel tRNA research aimed at understanding the cellular physiology and disease and development of tRNA-based therapeutics.
High-resolution quantitative profiling of tRNA abundance and ...
We overcome these limitations with modification-induced misincorporation tRNA sequencing (mim-tRNAseq), which combines a workflow for full-length cDNA library construction from endogenously modified tRNA with a comprehensive and …
Efficient and quantitative high-throughput tRNA sequencing
2015年7月27日 · High-throughput RNA sequencing (RNA-seq) has revolutionized our understanding of gene expression. Widely used RNA-seq methods start with adaptor ligation and cDNA synthesis of...
A robust method for measuring aminoacylation through tRNA-Seq …
2024年5月29日 · Here, we present an optimized charge tRNA-Seq method that combines previous developments with newly described approaches to establish a protocol for precise and accurate tRNA charge measurements. We verify that this protocol provides robust quantification of tRNA aminoacylation and we provide an end-to-end method that scales to hundreds of ...
Single-read tRNA-seq analysis reveals coordination of tRNA …
2022年12月20日 · Here, we develop a single-read tRNA analysis pipeline that takes advantage of the pseudo single-molecule nature of tRNA sequencing in NGS libraries. tRNAs are short enough that a single NGS read can represent one tRNA molecule, and can simultaneously report on the status of multiple modifications, aminoacylation, and fragmentation of each molecule.
Benchmarking tRNA-Seq quantification approaches by realistic tRNA-Seq …
2024年6月21日 · Here, we use realistic tRNA-Seq simulations to benchmark tRNA-Seq quantification approaches, including two novel approaches. We demonstrate that these novel approaches are consistently the most accurate, using data simulated to …
High-resolution quantitative profiling of tRNA abundance and ... - PubMed
Measurements of cellular tRNA abundance are hampered by pervasive blocks to cDNA synthesis at modified nucleosides and the extensive similarity among tRNA genes. We overcome these …
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