lac operator Sequence and Map - SnapGene
DNA sequence element that binds the lac repressor. Explore Over 2.7k Plasmids: Basic Cloning Vectors | More Plasmid Sets. This recognition sequence is asymmetric, so ligating blunt ends generated by BsrBI will not always regenerate a BsrBI site. BsrBI is typically used at 37°C, but can be used at temperatures up to 50°C.
The Nucleotide Sequence of the lac Operator - PMC - PubMed …
The lac repressor protects the lac operator against digestion with deoxyribonuclease. The protected fragment is double-stranded and about 27 base-pairs long. We determined the sequence of RNA transcription copies of this fragment and present a sequence for 24 base pairs.
The nucleotide sequence of the lac operator - PubMed
The lac repressor protects the lac operator against digestion with deoxyribonuclease. The protected fragment is double-stranded and about 27 base-pairs long. We determined the sequence of RNA transcription copies of this fragment and present a sequence for 24 base pairs.
12.1: The lac Operon - Biology LibreTexts
In the lac operon, these sequences are called P (promoter), O (operator), and CBS (CAP-binding site). Collectively, sequence elements such as these are called cis-elements because they must be located on the same piece of DNA as the genes they regulate.
Lac Operon - an overview | ScienceDirect Topics
Regulatory sequences of the lac operon. The sequence of only one strand of the control locus has been shown. Blue line above the sequence indicates the RNA polymerase-binding region. The central G:C base-pair in the lac operator is colored dark blue.
Structure and DNA sequence of the lac promoter and start sites…
The lactose (lac) operon promoter is positively regulated by the catabolite gene activator-cyclic AMP complex (CAP) that binds to the DNA located 61.5 bp upstream of the transcription start site....
carry the lac promoter-operator region. The lac repressor, when mixed with sonicated fragments of DNA from a phage that carries the lac genes, binds to a cel-lulose nitrate filter only those fragments containing the ( sopropyl-#,u-thiogalactoside, operator. IPTG a synthetic inducer of the lac operon) specifically elutes from the filter those fragme
DNA sequence of the lactose operon: the lacA gene and the ...
We report here the sequence of the lacA gene and the region distal to it, confirming the sequence of thiogalactoside transacetylase and completing the sequence of the lac operon. The lacA gene is characterized by use of rare codons, suggesting an origin …
Part:BBa K1624002 - parts.igem.org
Lac operator sequence, involved in transcriptional regulation of the lac operon. Can be placed between promoter and RBS sequence to regulate expression of gene by induction. Sequence from lacO in BL21(DE3) strain.
Genetic Regulation: The Lac Control Region | Science - AAAS
The nucleotide sequence of the lac promoter-operator region has been determined. The 122 base pairs comprising this region include the recognition sites for RNA polymerase, the positive regulatory protein, CAP, and the negative regulatory protein, the repressor.