【小鼠大学问】诱导型Cre，给基因敲除装个“开关” - 知乎
Mx1-Cre 小鼠在一般状态下不表达 Cre 重组酶，但可以通过干扰素-α、干扰素-β 或人工合成的双链 RNA类似物 poly I:C 的处理而诱导表达 Cre。 利用 Mx1-Cre 虽然可用于在发育过程中的任意时间点诱导目的基因敲除小鼠，但 仅限于 在响应干扰素的细胞中。
Cre-Lox recombination - Wikipedia
Cre-Lox recombination is a site-specific recombinase technology, used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus.
Cre Lox Breeding for Beginners, Part 1 | JAX Mice and Clinical …
The Cre/lox system is one of the most powerful and versatile tools developed for mouse genetics. It gives mouse researchers sophisticated control over the location and timing of gene expression. Cre/lox is usually used to make knockout alleles, but it …
Characterized cre lines - The Jackson Laboratory
GFAP-Cre transgenic mice from founder line 73.12 have Cre recombinase expression directed by the mouse glial fibrillary acidic protein promoter. Cre expression is observed in astrocytes in the brain and spinal cord, as well as postnatal and adult GFAP-expressing neural stem cells and their progeny in the brain.
Mouse Cre-LoxP system: general principles to determine tissue …
Dec 31, 2018 · A various tissue-specific Cre-driver mouse lines have been generated to date, and new Cre lines are still being developed. This review provides a brief overview of Cre- loxP system and a few commonly used promoters for expression of tissue-specific Cre recombinase.
Inducible Cre mice - PubMed
We will give an overview of available CreER (T2) transgenic mouse lines and present protocols that detail the generation of experimental mice for inducible gene knockout studies, the induction of recombination by tamoxifen treatment, and the analysis of the quality and quantity of recombination by reporter gene and target gene studies.
Cre Portal - The Jackson Laboratory
The Cre Portal contains curated data about all recombinase-containing transgenes and knock-ins developed in mice to provide a comprehensive resource delineating known recombinase activity patterns and allowing users to find relevant mouse resources for their studies.
Cre transgenic mouse lines - PubMed
The generation of conditional knockout mice is a multiple-step process, which involves mating the flox mutant mouse line (essential exon/s of the gene of interest are flanked by two LoxP sites) and the Cre-expressing mouse line.
Mouse Cre Models for the Study of Bone Diseases - PMC
The most widely used Cre transgenic mouse line for the in vivo study of osteoclasts is the CathepsinK (CtsK)-Cre knock-in (KI) mouse . In this line, selectivity of Cre expression in osteoclasts is thought to be reliable because the Cre gene is knocked into the CtsK gene locus, a well-accepted mature osteoclast marker gene.
A doxycycline- and light-inducible Cre recombinase mouse model …
Oct 28, 2022 · Together, we develop an efficient photoactivatable Cre recombinase mouse model and illustrate how this model is a powerful genome-editing tool for biological and biomedical research.
- Some results have been removed