The DeltaVision OMX V4 enables Total Internal Reflection Fluorescence Microscopy (TIRF). This technique uses an evanescent wave to selectively excite fluorophores that are close to the coverslip. This ...

The Wolfson Bioimaging Facility provides total internal reflection fluorescence microscopy (TIRF) with its multi-laser, dual-camera Olympus/Abbelight SAFe360 system which also offers single molecule ...

Besides GFP and two-photon, other fields of fluorescence microscopy have been greatly advanced in the last decade. For example, total internal reflectance fluorescence (TIRF), a form of excitation ...

Science presenter Jon Chase explains total internal reflection. He demonstrates how total internal reflection can make a coin disappear inside a glass of water and bend a laser beam. The second ...