pH is a measure of how acidic or alkaline a solution is. The diagram below shows what happens to an enzyme when denaturation occurs. Each enzyme has an optimum pH but it also has a working range ...

This investigation tests how well the enzyme amylase performs at different pH values. Amylase is a carbohydrase enzyme made by the pancreas and by glands in and around the mouth and throat.

Enzymes can be regulated by various means, such as allosteric regulation (binding of effector molecules), covalent modification (e.g., phosphorylation), and changes in pH or temperature. The Michaelis ...

Para-nitrophenol is yellow at alkaline pH and its concentration can be measured at 405 nm ... Prepare the substrate solution (3 tablets = 15 mg p-nitrophenylphosphate in 10 ml citrate buffer, = 5.5 mM ...

Whereas variation of pH, uni- or di-valent ion concentrations may affect the charge on both enzyme and substrate simultaneously, the addition of a water-insoluble amphipathic molecule to the lipid ...